Covid-19 Vaccine Coverage among People with Hiv in the Vaccine Safety Datalink

Background: People with HIV (PWH) may be at increased risk for severe COVID-19 outcomes compared with people without HIV. However, COVID-19 vaccination coverage among PWH is largely unknown, especially among those with advanced HIV or comorbidities. Method(s): We conducted a cohort study to evaluate coverage of the initial COVID-19 vaccine primary series and factors associated with the completion in adult PWH (>=18 years) enrolled in 8 healthcare organizations participating in the Vaccine Safety Datalink (VSD) project during December 1, 2020- December 31, 2021. Completion of two doses of the Pfizer-BioNTech or Moderna mRNA COVID-19 vaccines or one dose of the single-dose Janssen COVID-19 vaccine was assessed. Multivariable analysis was conducted using a robust Poisson regression model to estimate the rate ratio (RR) for factors associated with primary series completion, accounting for follow-up time. Result(s): A total of 22,063 PWH were identified, among which 89% were male and 93% were viral suppressed (viral load, VL <=200 copies/ml). Chronic comorbid conditions were prevalent, with 25% having a Charlson comorbidity score of 1-2 and 13% having a score of 3 or greater. About 23% were overweight and 17% were obese. The majority (90%) completed the primary series and 1,782 PWH (8%) did not receive any dose during the study period. A rapid uptake was achieved within the 6 months after the national COVID-19 vaccination program launched on December 14, 2020. (Figure 1) PWH who received one dose of mRNA vaccine (i.e., partially vaccinated) were excluded (n=314) from the analysis for the primary series completion. Having received an influenza vaccination in the past 2 years was the strongest predictor of completion (RR=1.17, 95%CI: 1.15, 1.20). Males (RR= 1.06, 95%CI: 1.04-1.08) and those of Asian race (RR=1.05, 95%CI: 1.03-1.06, vs. White) were more likely to complete the primary series. However, PWH with baseline CD4 counts < 200 (RR=0.97, 95%CI: 0.94-0.99) and those failing to achieve viral suppression (VL= 201-10k: RR= 0.89, 95%CI: 0.85-0.94;VL >10k: RR= 0.92, 95%CI: 0.87-0.98) were less likely to complete the primary series. Body mass index, Charlson comorbidity score, and neighborhood household income level were not associated with completion. Conclusion(s): Coverage of the COVID-19 vaccine primary series was high in adult PWH in the VSD. However, targeted vaccination outreach is warranted for PWH with low CD4 counts and uncontrolled HIV viral load.

Detection of Feline Coronavirus in Feline Effusions by Immunofluorescence Staining and Reverse Transcription Polymerase Chain Reaction.

Feline coronavirus (FCoV), the pathogen for feline infectious peritonitis, is a lethal infectious agent that can cause effusions in the pleural and abdominal cavities in domestic cats. To study the epidemiology of FCoV in Taiwan, 81 FIP-suspected sick cats with effusive specimens were recruited to test for FCoV infection using immunofluorescence staining and reverse transcription-polymerase chain reaction as detection methods, and viral RNAs were recovered from the specimens to conduct genotyping and phylogenetic analysis based on the spike (S) protein gene. The results revealed that a total of 47 (47/81, 58%) of the sick cats were positive for FCoV in the effusion samples, of which 39 were successfully sequenced and comprised of 21 type I strains, 9 type II strains, and 9 co-infections. The signalment analysis of these sick cats revealed that only the sex of cats showed a significant association (odds ratio = 2.74, 95% confidence interval = 1.06-7.07, p = 0.03) with the infection of FCoV, while age and breed showed no association. FCoV-positive cats demonstrated a significantly lower albumin to globulin ratio than negative individuals (p = 0.0004). The partial S gene-based phylogenetic analysis revealed that the type I strains demonstrated genetic diversity forming several clades, while the type II strains were more conserved. This study demonstrates the latest epidemiological status of FCoV infection in the northern part of Taiwan among sick cats and presents comparisons of Taiwan and other countries.

Mental Health During the COVID-19 Pandemic in Japan: Applying Topic Modeling in Daily Life Descriptions.

The novel coronavirus disease pandemic is threatening not only physical but also mental health. Although some recent quantitative studies have been conducted and revealed the influence of the pandemic on mental health and its relevant factors, it is impossible to obtain and explore all possible variables strongly related to mental health. Therefore, we attempted to adopt a bottom-up approach using text mining of participants' narratives. We examined how participants' descriptions of daily life during the pandemic were categorized into various topics, and which topics were related to their mental health in a sample of 776 Japanese citizens in the general population over 18 years old. Results of a topic modeling with 2,594 unique words provided nine topics (mask, physical symptoms, children, infection anxiety, disinfection items, economic influence, remote work, going out, and change of lifestyle). Those who wrote about economic influence, physical symptoms, and disinfection items experienced lower life satisfaction and higher depression and negative affect, whereas those who mentioned their children were likely to have higher life satisfaction. This study highlighted that monitoring the mental health of individuals with economic impacts and physical symptoms may reduce the damage of COVID-19. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s11469-021-00587-y.

US National Trends in Ophthalmic Procedures During the COVID-19 Pandemic

Purpose : To evaluate the relationship between COVID-19 case volume and ophthalmic procedural volume during the pandemic. Methods : A retrospective cohort study using TriNetX (Cambridge, MA, USA), a federated electronic health records research network comprising multiple large health organizations in the United States. Monthly Current Procedural Terminology (CPT)- specific volumes per HCO were clustered chronologically to calculate average volumes into three-month seasons to calculate average volumes. Seasonal averages from a combination of 2018 and 2019 data were used to provide a baseline comparison to pre-pandemic procedural volume. An aggregate of the total pandemic period (March 2020-August 2021) was compared to the corresponding figures in pre-pandemic timeframes. Results : 670,541 unique ophthalmic procedures from among 573 HCOs between March 2018 and August 2021 were included. Intravitreal injections was the most prevalent procedure with 320,106 occurrences. Phacoemulsification cataract surgery was the second most prevalent (N = 176,095) procedure with 144,816 uncomplicated (82.2%) and 31,279 complicated (17.8%). Intravitreal injections had the highest mean seasonal volume per HCO for each of the five COVID-19 pandemic seasons. From March 2020-August 2021, a mean pandemic volume of 266.7 (SD = 15) was observed, a 5% decrease (p<0.05) in procedures compared to pre-pandemic mean of 280.8 (SD = 26.1). During the five COVID-19 pandemic seasons, the seasonal mean volume almost always differed from pre-pandemic comparisons. Spring 2020 exhibited the sharpest seasonal decrease in procedural volume (88%). Spring 2021 had the largest count of significant increase in procedure volume (18%). Aggregate mean volume per HCO showed significant decreases for 11 out of 17 procedures in the 12-month March 2020-February 2021 timeframe and significant decreases for 10/17 procedures over the 18-month March 2020-August 2021 timeframe. A relative inverse relationship between COVID-19 case volume and ophthalmic procedure volume was observed. Conclusions : This study highlights the relative inverse relationship between COVID-19 cases and ophthalmic procedure volume in the US. Reduction in procedural volume may result in delayed care with potential for vision loss. Awareness and understanding of these trends could help ophthalmologists prepare should a similar cycle occur in the setting of the omicron and future variants.

Structure-guided antibody cocktail for prevention and treatment of COVID-19.

Development of effective therapeutics for mitigating the COVID-19 pandemic is a pressing global need. Neutralizing antibodies are known to be effective antivirals, as they can be rapidly deployed to prevent disease progression and can accelerate patient recovery without the need for fully developed host immunity. Here, we report the generation and characterization of a series of chimeric antibodies against the receptor-binding domain (RBD) of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike protein. Some of these antibodies exhibit exceptionally potent neutralization activities in vitro and in vivo, and the most potent of our antibodies target three distinct non-overlapping epitopes within the RBD. Cryo-electron microscopy analyses of two highly potent antibodies in complex with the SARS-CoV-2 spike protein suggested they may be particularly useful when combined in a cocktail therapy. The efficacy of this antibody cocktail was confirmed in SARS-CoV-2-infected mouse and hamster models as prophylactic and post-infection treatments. With the emergence of more contagious variants of SARS-CoV-2, cocktail antibody therapies hold great promise to control disease and prevent drug resistance.

Identification of COVID-19 B-cell epitopes with phage-displayed peptide library.

BACKGROUND: Coronavirus disease 19 (COVID-19) first appeared in the city of Wuhan, in the Hubei province of China. Since its emergence, the COVID-19-causing virus, SARS-CoV-2, has been rapidly transmitted around the globe, overwhelming the medical care systems in many countries and leading to more than 3.3 million deaths. Identification of immunological epitopes on the virus would be highly useful for the development of diagnostic tools and vaccines that will be critical to limiting further spread of COVID-19. METHODS: To find disease-specific B-cell epitopes that correspond to or mimic natural epitopes, we used phage display technology to determine the targets of specific antibodies present in the sera of immune-responsive COVID-19 patients. Enzyme-linked immunosorbent assays were further applied to assess competitive antibody binding and serological detection. VaxiJen, BepiPred-2.0 and DiscoTope 2.0 were utilized for B-cell epitope prediction. PyMOL was used for protein structural analysis. RESULTS: 36 enriched peptides were identified by biopanning with antibodies from two COVID-19 patients; the peptides 4 motifs with consensus residues corresponding to two potential B-cell epitopes on SARS-CoV-2 viral proteins. The putative epitopes and hit peptides were then synthesized for validation by competitive antibody binding and serological detection. CONCLUSIONS: The identified B-cell epitopes on SARS-CoV-2 may aid investigations into COVID-19 pathogenesis and facilitate the development of epitope-based serological diagnostics and vaccines.

The Ongoing Global COVID-19 Pandemic and Related Vaccines

Since the outbreak of COVID-19 in Wuhan, China in late 2019 through April 2021, 140 million people have been infected and 3 million people have died from the pandemic worldwide. In the United States, more than 30 million people have been tested positively which caused more than 560,000 death. Two dozen countries have reported more than one million confirmed cases within their borders. Nowadays, approximately 300 million vaccine doses have been administered in the U.S. About 150 million Americans have received at least one dose. The 9.33-million-people Israel enjoys the highest rate of COVID-19 vaccination in the world after nearly 9% of her population infected and more than 6,400 died. This can be contributed to its limited population and enormous influence in Europe and America. The United States enacted Vaccine Act as early as 1813. The federal and state governments have many kinds of regulations of vaccine medicine injury compensation. The relevant provisions may be found in different laws, including Title 42 of the U.S. Code: The Public Health and Welfare.

Temporal distancing during the COVID-19 pandemic: Letter writing with future self can mitigate negative affect.

Novel coronavirus disease (COVID-19) is spreading across the world, threatening not only physical health but also psychological well-being. We reasoned that a broadened temporal perspective may attenuate current mental distress and tested a letter-writing manipulation designed to connect people to their post-COVID-19 future selves. We conducted an online experiment with 738 Japanese participants recruited from two common survey platforms. They were randomly assigned to either send a letter to their future self (letter-to-future) condition, send a letter to present self from the perspective of future self (letter-from-future) condition, or a control condition. Participants in both letter-writing conditions showed immediate decrease in negative affect and increase in positive affect relative to the control condition. These effects were mediated by temporal distancing from the current situation. These findings suggest that taking a broader temporal perspective can be achieved by letter writing with a future self and may offer an effective means of regulating negative affect in a stressful present time such as the COVID-19 pandemic.

The impact of COVID-19 pandemic on high performance secondary school student-athletes

Purpose: The COVID-19 pandemic has led to a worldwide disruption of the sporting industry. Secondary school student-athletes, as a distinct population, are facing unique social and academic challenges. It is important to identify some of the unique challenges this population currently faces, and understand where our student-athletes are at mentally and physically. This is in order to ensure their needs are addressed, and the health and wellbeing of this population is protected. This study aimed to assess the impact of the COVID-19 pandemic on Canadian high performance secondary school student-athletes.